TransScript一步法去除 gDNA及第一鏈 cDNA 合成試劑盒-AT311
產(chǎn)品名稱
TransScript? One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311)
應(yīng)用專利
專利號：ZL201710031139.6
高分文獻(xiàn)集錦
?Gong Q, Wang Y, He L, et al. Molecular basis of methyl-salicylate-mediated plant airborne defence[J]. Nature, 2023.(IF 64.80)
?Guan J, Wang G, Wang J, et al. Chemical reprogramming of human somatic cells to pluripotent stem cells[J]. Nature, 2022.(IF 49.00)
?Wang J Z, Chen T Y, Zhang Z D, et al. Remodelling autoactive NLRs for broad-spectrum immunity in plants [J]. Nature, 2025.(IF 48.50)
?Wang S, Du Y, Zhang B, et al. Transplantation of chemically induced pluripotent stem-cell-derived islets under abdominal anterior rectus sheath in a type 1 diabetes patient[J]. Cell, 2024.(IF 45.50)
?Zhao P Z, Yang H, Sun J Y, et al. Targeted MYC2 stabilization confers citrus Huanglongbing resistance [J]. Science, 2025.(IF 44.70)
?Chen J, Ou Y, Luo R, et al. SAR1B senses leucine levels to regulate mTORC1 signalling[J]. Nature, 2021.(IF 42.77)
?Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018.(IF 40.13)
?Zhao K, Xue H, Li G, et al. Pangenome analysis reveals structural variation associated with seed size and weight traits in peanut[J]. Nature genetics, 2025.(IF 31.80)
?Liu S, Liu C, Lv X, et al. The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis[J]. Immunity, 2021.(IF 31.74)
?Huang L, Wei M, Li H, et al. GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis[J]. Molecular Cancer, 2025.(IF 27.70)
?Fan H, Quan S, Ye Q, et al. A molecular framework underlying low-nitrogen-induced early leaf senescence in Arabidopsis thaliana[J]. Molecular Plant, 2023.(IF 27.50)
?Zhu J, Zhong X, He H, et al. Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation[J]. Cell Stem Cell, 2024.(IF 19.80)
?Wang R, Xue Y, Fan J, et al. A systems genetics approach reveals PbrNSC as a regulator of lignin and cellulose biosynthesis in stone cells of pear fruit[J]. Genome Biology, 2021.(IF 17.90)
?Zhang Y, Chen W, Wu D, et al. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli[J]. Nucleic acids research, 2022.(IF 16.97)
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產(chǎn)品特點(diǎn)
? 在同一反應(yīng)體系中，同時完成反轉(zhuǎn)錄與基因組 DNA 的去除，操作簡便，降低污染機(jī)率。
? 產(chǎn)物用于 qPCR：反轉(zhuǎn)錄 15 分鐘；產(chǎn)物用于 PCR：反轉(zhuǎn)錄 30 分鐘。
? 反應(yīng)結(jié)束后，同時熱失活 RT/RI 與 gDNA Remover。
? 合成片段 ≤12 kb。
數(shù)據(jù)展示
PCR 檢測
qPCR 檢測
以人、煙草 RNA 為模板，分別使用 TransGen 和 Company V 產(chǎn)品進(jìn)行反轉(zhuǎn)錄，PCR (AS131) 和 qPCR (AQ601) 檢測反轉(zhuǎn)錄效果。結(jié)果表明， TransGen 產(chǎn)品反轉(zhuǎn)錄效果更好。
產(chǎn)品信息
